cd1 igs female mice  (Charles River Laboratories)

Journal: Pharmaceutics

Article Title: Toxicity, Half-Life and Antitumor Activity of Phenyl 4-(2-Oxo-3-alkylimidazolidin-1-yl)benzenesulfonates as Novel Antimitotic CYP1A1-Targeted Prodrugs in Female Mouse Models

doi: 10.3390/pharmaceutics17020233

Figure Lengend Snippet: Representative macroscopic pictures of livers, kidneys, and lungs of CD1 ® IGS female mice necropsied after multi-intravenous administration of NaCl 0.9%, vehicle, CEU-835, -934, and -938. The scale bar is set at 1000 mm. n = 3.

Article Snippet: CD1 ® IGS female mice 16–18 g ( https://www.criver.com/products-services/find-model/cd-1r-igs-mouse?region=24 , accessed on 20 December 2024) and Nude CD-1 female mice CD1- Foxn1 nu 18–23 g ( https://www.criver.com/products-services/find-model/cd-1-nude-mouse?region=24 , accessed on 20 December 2024) were purchased from Charles River Laboratories Canada (Laval, QC, Canada).

Techniques:

Journal: Pharmaceutics

Article Title: Toxicity, Half-Life and Antitumor Activity of Phenyl 4-(2-Oxo-3-alkylimidazolidin-1-yl)benzenesulfonates as Novel Antimitotic CYP1A1-Targeted Prodrugs in Female Mouse Models

doi: 10.3390/pharmaceutics17020233

Figure Lengend Snippet: Total blood count profiles after multi-intravenous administration of NaCl 0.9% ( n = 3), vehicle ( n = 3), CEU-835 ( n = 3), -934 ( n = 3), and -938 ( n = 3) in CD1 ® IGS female mice of ( A ) white blood cells, ( B ) lymphocytes, ( C ) monocytes, ( D ) granulocytes, ( E ) eosinophils, ( F ) red blood cells, ( G ) hemoglobin, ( H ) hematocrit, ( I ) mean corpuscular volume, ( J ) mean corpuscular hemoglobin, ( K ) mean corpuscular hemoglobin concentration, ( L ) red cell distribution width, ( M ) platelet count, and ( N ) mean platelet volume. The p -value for all parameters is p < 0.05. ns: non-statistically significant. *: statistically significant.

Article Snippet: CD1 ® IGS female mice 16–18 g ( https://www.criver.com/products-services/find-model/cd-1r-igs-mouse?region=24 , accessed on 20 December 2024) and Nude CD-1 female mice CD1- Foxn1 nu 18–23 g ( https://www.criver.com/products-services/find-model/cd-1-nude-mouse?region=24 , accessed on 20 December 2024) were purchased from Charles River Laboratories Canada (Laval, QC, Canada).

Techniques: Concentration Assay

Journal: Pharmaceutics

Article Title: Toxicity, Half-Life and Antitumor Activity of Phenyl 4-(2-Oxo-3-alkylimidazolidin-1-yl)benzenesulfonates as Novel Antimitotic CYP1A1-Targeted Prodrugs in Female Mouse Models

doi: 10.3390/pharmaceutics17020233

Figure Lengend Snippet: Representative pictures of the eosin-hematoxylin histological analysis at 10× of liver, kidneys, and lungs of CD1 ® IGS female mice necropsied after multi-intravenous administration of NaCl 0.9%, vehicle, CEU-835, -934, and -938. The scale bar is set at 300 µm. The indicated tissue structures are blood vessels (red arrows), glomeruli (blue arrows), renal tubulointerstitium (green arrows), parenchyma (light blue arrows) and bronchoalveolar structures (black arrows). n = 3.

Article Snippet: CD1 ® IGS female mice 16–18 g ( https://www.criver.com/products-services/find-model/cd-1r-igs-mouse?region=24 , accessed on 20 December 2024) and Nude CD-1 female mice CD1- Foxn1 nu 18–23 g ( https://www.criver.com/products-services/find-model/cd-1-nude-mouse?region=24 , accessed on 20 December 2024) were purchased from Charles River Laboratories Canada (Laval, QC, Canada).

Techniques:

Journal: Molecular Oncology

Article Title: PIKFYVE inhibitors trigger interleukin‐24‐dependent cell death of autophagy‐dependent melanoma

doi: 10.1002/1878-0261.13607

Figure Lengend Snippet: WX8 inhibited formation and growth of melanoma tumors. Melanoma A375 cells were cultured for 8 h with either vehicle (A) or 10 μ m WX8 (B) to inhibit PIKFYVE effectively (extensive cytoplasmic vacuolation) while retaining cell viability (resistance to trypan blue). Bar indicates 20 μm. Cytoplasmic vacuolization occurred only in the WX8‐treated cells. (C) Nude mice ( n = 5) were immediately inoculated in their left flank with cells pretreated with vehicle and in their right flank with cells pretreated with WX8. Tumors were excised at 28 days. Ruler is in cm. (D) Periodic measurements of tumor volume and mouse weight revealed that cells pretreated with vehicle (open circles) formed tumors more rapidly than cells pretreated with WX8 (solid circles). Mouse weight gradually increased (shaded squares, ± SEM, n = 5). (E) To evaluate WX8 toxicity, CD1 IGS mice received daily intraperitoneal injections of either vehicle or WX8. Mice were weighed each day (±SD, n = 2). (F) Outbred homozygous nude mice were inoculated on both flanks with melanoma A375 cells and then palpable tumors were allowed to form. Tumors were excised at 21 days. Bar indicates 1 cm. (G) Mice with palpable tumors received daily intraperitoneal injections of either vehicle or WX8 and tumor volume quantified. (H) Mice in panel G were weighed each day (± SEM, n = 3, for each treatment). Images in panels A–C are representative of three samples.

Article Snippet: Female CD1 IGS [Crl:CD1(ICR)] mice 6–8 weeks old (Charles River) were used for toxicity tests.

Techniques: Cell Culture